program 43 Search Results


anti aif rabbit polyclonal antibody  (ProSci Incorporated)
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ProSci Incorporated anti aif rabbit polyclonal antibody
Figure 1 Apaf1-deficiency leads to accumulation of NPCs expressing neural markers in vivo and in vitro. (a) BrdU staining on coronal sections from e12.5 wt and Apaf1/ (/) littermates embryos. nt: neural tube (hindbrain), fb: forebrain. (b) Analysis of differentiation markers from e14 wt and Apaf1/ littermate embryos. nestin, delta1, notch1 mRNA levels were revealed by in situ mRNA hybridization as previously described (Stoykova and Gruss, 1994) on embryonic coronal sections. Class III b- tubulin was revealed by immunohistochemistry on embryonic e14.5 coronal sections with a mouse monoclonal anti-class III b-tubulin antibody. The corresponding histological structures are indicated in the sketch and in the right panel as follows: I: intermediate zone, M: marginal zone, V: ventricular germinal zone, ve: brain ventricle. Scale bar: 0.4 mm. (c) ETNA þ / þ ( þ / þ ) and ETNA/ (/) cell lines were isolated from e14 wt and mutant embryos, respectively, and immortalized with a retrovirus transducing the tsA58/U19 large T antigen. Total extracts from both cell lines were immunoprecipitated with an anti-Apaf1 rabbit <t>polyclonal</t> antibody and analysed with a rat monoclonal antibody anti-Apaf1. Ctrl: human neuroblastoma cells (SH-SY5Y) stably overexpressing Apaf1. (d) Total cell lysates from ETNA þ / þ and ETNA/ cells, either naı¨ve (N) or incubated for 48 h with a differentiation medium (D), were analysed by Western blotting for the expression of NeuN, class III b-tubulin, ChAT, PSD95, tau; b-actin was also assayed as a control of equal protein loading. (e) ETNA þ / þ and ETNA/ cells, either naı¨ve or differentiated, were immunostained with an antibody recognizing the 160 kDa form of neurofilament (NF-M). Scale bar: 40 mm
Anti Aif Rabbit Polyclonal Antibody, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cl communitytm version 3.43  (ChunLab Inc)
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ChunLab Inc cl communitytm version 3.43
Figure 1 Apaf1-deficiency leads to accumulation of NPCs expressing neural markers in vivo and in vitro. (a) BrdU staining on coronal sections from e12.5 wt and Apaf1/ (/) littermates embryos. nt: neural tube (hindbrain), fb: forebrain. (b) Analysis of differentiation markers from e14 wt and Apaf1/ littermate embryos. nestin, delta1, notch1 mRNA levels were revealed by in situ mRNA hybridization as previously described (Stoykova and Gruss, 1994) on embryonic coronal sections. Class III b- tubulin was revealed by immunohistochemistry on embryonic e14.5 coronal sections with a mouse monoclonal anti-class III b-tubulin antibody. The corresponding histological structures are indicated in the sketch and in the right panel as follows: I: intermediate zone, M: marginal zone, V: ventricular germinal zone, ve: brain ventricle. Scale bar: 0.4 mm. (c) ETNA þ / þ ( þ / þ ) and ETNA/ (/) cell lines were isolated from e14 wt and mutant embryos, respectively, and immortalized with a retrovirus transducing the tsA58/U19 large T antigen. Total extracts from both cell lines were immunoprecipitated with an anti-Apaf1 rabbit <t>polyclonal</t> antibody and analysed with a rat monoclonal antibody anti-Apaf1. Ctrl: human neuroblastoma cells (SH-SY5Y) stably overexpressing Apaf1. (d) Total cell lysates from ETNA þ / þ and ETNA/ cells, either naı¨ve (N) or incubated for 48 h with a differentiation medium (D), were analysed by Western blotting for the expression of NeuN, class III b-tubulin, ChAT, PSD95, tau; b-actin was also assayed as a control of equal protein loading. (e) ETNA þ / þ and ETNA/ cells, either naı¨ve or differentiated, were immunostained with an antibody recognizing the 160 kDa form of neurofilament (NF-M). Scale bar: 40 mm
Cl Communitytm Version 3.43, supplied by ChunLab Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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labview-based program v1.43 chep  (ALEA Solutions GmbH)
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ALEA Solutions GmbH labview-based program v1.43 chep
Figure 1 Apaf1-deficiency leads to accumulation of NPCs expressing neural markers in vivo and in vitro. (a) BrdU staining on coronal sections from e12.5 wt and Apaf1/ (/) littermates embryos. nt: neural tube (hindbrain), fb: forebrain. (b) Analysis of differentiation markers from e14 wt and Apaf1/ littermate embryos. nestin, delta1, notch1 mRNA levels were revealed by in situ mRNA hybridization as previously described (Stoykova and Gruss, 1994) on embryonic coronal sections. Class III b- tubulin was revealed by immunohistochemistry on embryonic e14.5 coronal sections with a mouse monoclonal anti-class III b-tubulin antibody. The corresponding histological structures are indicated in the sketch and in the right panel as follows: I: intermediate zone, M: marginal zone, V: ventricular germinal zone, ve: brain ventricle. Scale bar: 0.4 mm. (c) ETNA þ / þ ( þ / þ ) and ETNA/ (/) cell lines were isolated from e14 wt and mutant embryos, respectively, and immortalized with a retrovirus transducing the tsA58/U19 large T antigen. Total extracts from both cell lines were immunoprecipitated with an anti-Apaf1 rabbit <t>polyclonal</t> antibody and analysed with a rat monoclonal antibody anti-Apaf1. Ctrl: human neuroblastoma cells (SH-SY5Y) stably overexpressing Apaf1. (d) Total cell lysates from ETNA þ / þ and ETNA/ cells, either naı¨ve (N) or incubated for 48 h with a differentiation medium (D), were analysed by Western blotting for the expression of NeuN, class III b-tubulin, ChAT, PSD95, tau; b-actin was also assayed as a control of equal protein loading. (e) ETNA þ / þ and ETNA/ cells, either naı¨ve or differentiated, were immunostained with an antibody recognizing the 160 kDa form of neurofilament (NF-M). Scale bar: 40 mm
Labview Based Program V1.43 Chep, supplied by ALEA Solutions GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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crysalis version 171.38.43 package of programs  (Rigaku Corporation)
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Rigaku Corporation crysalis version 171.38.43 package of programs
Figure 1 Apaf1-deficiency leads to accumulation of NPCs expressing neural markers in vivo and in vitro. (a) BrdU staining on coronal sections from e12.5 wt and Apaf1/ (/) littermates embryos. nt: neural tube (hindbrain), fb: forebrain. (b) Analysis of differentiation markers from e14 wt and Apaf1/ littermate embryos. nestin, delta1, notch1 mRNA levels were revealed by in situ mRNA hybridization as previously described (Stoykova and Gruss, 1994) on embryonic coronal sections. Class III b- tubulin was revealed by immunohistochemistry on embryonic e14.5 coronal sections with a mouse monoclonal anti-class III b-tubulin antibody. The corresponding histological structures are indicated in the sketch and in the right panel as follows: I: intermediate zone, M: marginal zone, V: ventricular germinal zone, ve: brain ventricle. Scale bar: 0.4 mm. (c) ETNA þ / þ ( þ / þ ) and ETNA/ (/) cell lines were isolated from e14 wt and mutant embryos, respectively, and immortalized with a retrovirus transducing the tsA58/U19 large T antigen. Total extracts from both cell lines were immunoprecipitated with an anti-Apaf1 rabbit <t>polyclonal</t> antibody and analysed with a rat monoclonal antibody anti-Apaf1. Ctrl: human neuroblastoma cells (SH-SY5Y) stably overexpressing Apaf1. (d) Total cell lysates from ETNA þ / þ and ETNA/ cells, either naı¨ve (N) or incubated for 48 h with a differentiation medium (D), were analysed by Western blotting for the expression of NeuN, class III b-tubulin, ChAT, PSD95, tau; b-actin was also assayed as a control of equal protein loading. (e) ETNA þ / þ and ETNA/ cells, either naı¨ve or differentiated, were immunostained with an antibody recognizing the 160 kDa form of neurofilament (NF-M). Scale bar: 40 mm
Crysalis Version 171.38.43 Package Of Programs, supplied by Rigaku Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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crysalis version 171.38.43 package of programs - by Bioz Stars, 2026-06
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crysalice pro 171.39.46e software  (Rigaku Corporation)
90
Rigaku Corporation crysalice pro 171.39.46e software
Figure 1 Apaf1-deficiency leads to accumulation of NPCs expressing neural markers in vivo and in vitro. (a) BrdU staining on coronal sections from e12.5 wt and Apaf1/ (/) littermates embryos. nt: neural tube (hindbrain), fb: forebrain. (b) Analysis of differentiation markers from e14 wt and Apaf1/ littermate embryos. nestin, delta1, notch1 mRNA levels were revealed by in situ mRNA hybridization as previously described (Stoykova and Gruss, 1994) on embryonic coronal sections. Class III b- tubulin was revealed by immunohistochemistry on embryonic e14.5 coronal sections with a mouse monoclonal anti-class III b-tubulin antibody. The corresponding histological structures are indicated in the sketch and in the right panel as follows: I: intermediate zone, M: marginal zone, V: ventricular germinal zone, ve: brain ventricle. Scale bar: 0.4 mm. (c) ETNA þ / þ ( þ / þ ) and ETNA/ (/) cell lines were isolated from e14 wt and mutant embryos, respectively, and immortalized with a retrovirus transducing the tsA58/U19 large T antigen. Total extracts from both cell lines were immunoprecipitated with an anti-Apaf1 rabbit <t>polyclonal</t> antibody and analysed with a rat monoclonal antibody anti-Apaf1. Ctrl: human neuroblastoma cells (SH-SY5Y) stably overexpressing Apaf1. (d) Total cell lysates from ETNA þ / þ and ETNA/ cells, either naı¨ve (N) or incubated for 48 h with a differentiation medium (D), were analysed by Western blotting for the expression of NeuN, class III b-tubulin, ChAT, PSD95, tau; b-actin was also assayed as a control of equal protein loading. (e) ETNA þ / þ and ETNA/ cells, either naı¨ve or differentiated, were immunostained with an antibody recognizing the 160 kDa form of neurofilament (NF-M). Scale bar: 40 mm
Crysalice Pro 171.39.46e Software, supplied by Rigaku Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mothur program v.1.43.0  (RStudio)
90
RStudio mothur program v.1.43.0
Figure 1 Apaf1-deficiency leads to accumulation of NPCs expressing neural markers in vivo and in vitro. (a) BrdU staining on coronal sections from e12.5 wt and Apaf1/ (/) littermates embryos. nt: neural tube (hindbrain), fb: forebrain. (b) Analysis of differentiation markers from e14 wt and Apaf1/ littermate embryos. nestin, delta1, notch1 mRNA levels were revealed by in situ mRNA hybridization as previously described (Stoykova and Gruss, 1994) on embryonic coronal sections. Class III b- tubulin was revealed by immunohistochemistry on embryonic e14.5 coronal sections with a mouse monoclonal anti-class III b-tubulin antibody. The corresponding histological structures are indicated in the sketch and in the right panel as follows: I: intermediate zone, M: marginal zone, V: ventricular germinal zone, ve: brain ventricle. Scale bar: 0.4 mm. (c) ETNA þ / þ ( þ / þ ) and ETNA/ (/) cell lines were isolated from e14 wt and mutant embryos, respectively, and immortalized with a retrovirus transducing the tsA58/U19 large T antigen. Total extracts from both cell lines were immunoprecipitated with an anti-Apaf1 rabbit <t>polyclonal</t> antibody and analysed with a rat monoclonal antibody anti-Apaf1. Ctrl: human neuroblastoma cells (SH-SY5Y) stably overexpressing Apaf1. (d) Total cell lysates from ETNA þ / þ and ETNA/ cells, either naı¨ve (N) or incubated for 48 h with a differentiation medium (D), were analysed by Western blotting for the expression of NeuN, class III b-tubulin, ChAT, PSD95, tau; b-actin was also assayed as a control of equal protein loading. (e) ETNA þ / þ and ETNA/ cells, either naı¨ve or differentiated, were immunostained with an antibody recognizing the 160 kDa form of neurofilament (NF-M). Scale bar: 40 mm
Mothur Program V.1.43.0, supplied by RStudio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tech-43 fuel/geoc technical program  (Loews Corporation)
90
Loews Corporation tech-43 fuel/geoc technical program
Figure 1 Apaf1-deficiency leads to accumulation of NPCs expressing neural markers in vivo and in vitro. (a) BrdU staining on coronal sections from e12.5 wt and Apaf1/ (/) littermates embryos. nt: neural tube (hindbrain), fb: forebrain. (b) Analysis of differentiation markers from e14 wt and Apaf1/ littermate embryos. nestin, delta1, notch1 mRNA levels were revealed by in situ mRNA hybridization as previously described (Stoykova and Gruss, 1994) on embryonic coronal sections. Class III b- tubulin was revealed by immunohistochemistry on embryonic e14.5 coronal sections with a mouse monoclonal anti-class III b-tubulin antibody. The corresponding histological structures are indicated in the sketch and in the right panel as follows: I: intermediate zone, M: marginal zone, V: ventricular germinal zone, ve: brain ventricle. Scale bar: 0.4 mm. (c) ETNA þ / þ ( þ / þ ) and ETNA/ (/) cell lines were isolated from e14 wt and mutant embryos, respectively, and immortalized with a retrovirus transducing the tsA58/U19 large T antigen. Total extracts from both cell lines were immunoprecipitated with an anti-Apaf1 rabbit <t>polyclonal</t> antibody and analysed with a rat monoclonal antibody anti-Apaf1. Ctrl: human neuroblastoma cells (SH-SY5Y) stably overexpressing Apaf1. (d) Total cell lysates from ETNA þ / þ and ETNA/ cells, either naı¨ve (N) or incubated for 48 h with a differentiation medium (D), were analysed by Western blotting for the expression of NeuN, class III b-tubulin, ChAT, PSD95, tau; b-actin was also assayed as a control of equal protein loading. (e) ETNA þ / þ and ETNA/ cells, either naı¨ve or differentiated, were immunostained with an antibody recognizing the 160 kDa form of neurofilament (NF-M). Scale bar: 40 mm
Tech 43 Fuel/Geoc Technical Program, supplied by Loews Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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power and sample size program version 3.0.43  (OriginLab corp)
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OriginLab corp power and sample size program version 3.0.43
Figure 1 Apaf1-deficiency leads to accumulation of NPCs expressing neural markers in vivo and in vitro. (a) BrdU staining on coronal sections from e12.5 wt and Apaf1/ (/) littermates embryos. nt: neural tube (hindbrain), fb: forebrain. (b) Analysis of differentiation markers from e14 wt and Apaf1/ littermate embryos. nestin, delta1, notch1 mRNA levels were revealed by in situ mRNA hybridization as previously described (Stoykova and Gruss, 1994) on embryonic coronal sections. Class III b- tubulin was revealed by immunohistochemistry on embryonic e14.5 coronal sections with a mouse monoclonal anti-class III b-tubulin antibody. The corresponding histological structures are indicated in the sketch and in the right panel as follows: I: intermediate zone, M: marginal zone, V: ventricular germinal zone, ve: brain ventricle. Scale bar: 0.4 mm. (c) ETNA þ / þ ( þ / þ ) and ETNA/ (/) cell lines were isolated from e14 wt and mutant embryos, respectively, and immortalized with a retrovirus transducing the tsA58/U19 large T antigen. Total extracts from both cell lines were immunoprecipitated with an anti-Apaf1 rabbit <t>polyclonal</t> antibody and analysed with a rat monoclonal antibody anti-Apaf1. Ctrl: human neuroblastoma cells (SH-SY5Y) stably overexpressing Apaf1. (d) Total cell lysates from ETNA þ / þ and ETNA/ cells, either naı¨ve (N) or incubated for 48 h with a differentiation medium (D), were analysed by Western blotting for the expression of NeuN, class III b-tubulin, ChAT, PSD95, tau; b-actin was also assayed as a control of equal protein loading. (e) ETNA þ / þ and ETNA/ cells, either naı¨ve or differentiated, were immunostained with an antibody recognizing the 160 kDa form of neurofilament (NF-M). Scale bar: 40 mm
Power And Sample Size Program Version 3.0.43, supplied by OriginLab corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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program 43  (Molecular Dynamics Inc)
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Molecular Dynamics Inc program 43
Figure 1 Apaf1-deficiency leads to accumulation of NPCs expressing neural markers in vivo and in vitro. (a) BrdU staining on coronal sections from e12.5 wt and Apaf1/ (/) littermates embryos. nt: neural tube (hindbrain), fb: forebrain. (b) Analysis of differentiation markers from e14 wt and Apaf1/ littermate embryos. nestin, delta1, notch1 mRNA levels were revealed by in situ mRNA hybridization as previously described (Stoykova and Gruss, 1994) on embryonic coronal sections. Class III b- tubulin was revealed by immunohistochemistry on embryonic e14.5 coronal sections with a mouse monoclonal anti-class III b-tubulin antibody. The corresponding histological structures are indicated in the sketch and in the right panel as follows: I: intermediate zone, M: marginal zone, V: ventricular germinal zone, ve: brain ventricle. Scale bar: 0.4 mm. (c) ETNA þ / þ ( þ / þ ) and ETNA/ (/) cell lines were isolated from e14 wt and mutant embryos, respectively, and immortalized with a retrovirus transducing the tsA58/U19 large T antigen. Total extracts from both cell lines were immunoprecipitated with an anti-Apaf1 rabbit <t>polyclonal</t> antibody and analysed with a rat monoclonal antibody anti-Apaf1. Ctrl: human neuroblastoma cells (SH-SY5Y) stably overexpressing Apaf1. (d) Total cell lysates from ETNA þ / þ and ETNA/ cells, either naı¨ve (N) or incubated for 48 h with a differentiation medium (D), were analysed by Western blotting for the expression of NeuN, class III b-tubulin, ChAT, PSD95, tau; b-actin was also assayed as a control of equal protein loading. (e) ETNA þ / þ and ETNA/ cells, either naı¨ve or differentiated, were immunostained with an antibody recognizing the 160 kDa form of neurofilament (NF-M). Scale bar: 40 mm
Program 43, supplied by Molecular Dynamics Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 1 Apaf1-deficiency leads to accumulation of NPCs expressing neural markers in vivo and in vitro. (a) BrdU staining on coronal sections from e12.5 wt and Apaf1/ (/) littermates embryos. nt: neural tube (hindbrain), fb: forebrain. (b) Analysis of differentiation markers from e14 wt and Apaf1/ littermate embryos. nestin, delta1, notch1 mRNA levels were revealed by in situ mRNA hybridization as previously described (Stoykova and Gruss, 1994) on embryonic coronal sections. Class III b- tubulin was revealed by immunohistochemistry on embryonic e14.5 coronal sections with a mouse monoclonal anti-class III b-tubulin antibody. The corresponding histological structures are indicated in the sketch and in the right panel as follows: I: intermediate zone, M: marginal zone, V: ventricular germinal zone, ve: brain ventricle. Scale bar: 0.4 mm. (c) ETNA þ / þ ( þ / þ ) and ETNA/ (/) cell lines were isolated from e14 wt and mutant embryos, respectively, and immortalized with a retrovirus transducing the tsA58/U19 large T antigen. Total extracts from both cell lines were immunoprecipitated with an anti-Apaf1 rabbit polyclonal antibody and analysed with a rat monoclonal antibody anti-Apaf1. Ctrl: human neuroblastoma cells (SH-SY5Y) stably overexpressing Apaf1. (d) Total cell lysates from ETNA þ / þ and ETNA/ cells, either naı¨ve (N) or incubated for 48 h with a differentiation medium (D), were analysed by Western blotting for the expression of NeuN, class III b-tubulin, ChAT, PSD95, tau; b-actin was also assayed as a control of equal protein loading. (e) ETNA þ / þ and ETNA/ cells, either naı¨ve or differentiated, were immunostained with an antibody recognizing the 160 kDa form of neurofilament (NF-M). Scale bar: 40 mm

Journal: Cell death and differentiation

Article Title: Apoptosome inactivation rescues proneural and neural cells from neurodegeneration.

doi: 10.1038/sj.cdd.4401476

Figure Lengend Snippet: Figure 1 Apaf1-deficiency leads to accumulation of NPCs expressing neural markers in vivo and in vitro. (a) BrdU staining on coronal sections from e12.5 wt and Apaf1/ (/) littermates embryos. nt: neural tube (hindbrain), fb: forebrain. (b) Analysis of differentiation markers from e14 wt and Apaf1/ littermate embryos. nestin, delta1, notch1 mRNA levels were revealed by in situ mRNA hybridization as previously described (Stoykova and Gruss, 1994) on embryonic coronal sections. Class III b- tubulin was revealed by immunohistochemistry on embryonic e14.5 coronal sections with a mouse monoclonal anti-class III b-tubulin antibody. The corresponding histological structures are indicated in the sketch and in the right panel as follows: I: intermediate zone, M: marginal zone, V: ventricular germinal zone, ve: brain ventricle. Scale bar: 0.4 mm. (c) ETNA þ / þ ( þ / þ ) and ETNA/ (/) cell lines were isolated from e14 wt and mutant embryos, respectively, and immortalized with a retrovirus transducing the tsA58/U19 large T antigen. Total extracts from both cell lines were immunoprecipitated with an anti-Apaf1 rabbit polyclonal antibody and analysed with a rat monoclonal antibody anti-Apaf1. Ctrl: human neuroblastoma cells (SH-SY5Y) stably overexpressing Apaf1. (d) Total cell lysates from ETNA þ / þ and ETNA/ cells, either naı¨ve (N) or incubated for 48 h with a differentiation medium (D), were analysed by Western blotting for the expression of NeuN, class III b-tubulin, ChAT, PSD95, tau; b-actin was also assayed as a control of equal protein loading. (e) ETNA þ / þ and ETNA/ cells, either naı¨ve or differentiated, were immunostained with an antibody recognizing the 160 kDa form of neurofilament (NF-M). Scale bar: 40 mm

Article Snippet: We used an anti-cytochrome c mouse monoclonal antibody (clone 6H2.B4, BD PharMingen), an anti-cytochrome c rabbit polyclonal antibody (Santa Cruz), an anti-AIF rabbit polyclonal antibody (a generous gift of Guido Kroemer), an anti-EndoG rabbit polyclonal antibody (ProSci Incorporated), an anti-Neurofilament 160 mouse monoclonal antibody (clone NN18, SIGMA), anti-Apaf1 polyclonal antibody (AB16941, Chemicon Int.) and an anti- Large T Antigen monoclonal antibody (clone PAb419, Oncogene).

Techniques: Expressing, In Vivo, In Vitro, BrdU Staining, In Situ, Hybridization, Immunohistochemistry, Isolation, Mutagenesis, Immunoprecipitation, Stable Transfection, Incubation, Western Blot, Control